Coding

Part:BBa_K156000:Design

Designed by: George Washington   Group: iGEM08_Virginia   (2008-06-16)


PoPS->PhaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 886
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 852
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 237
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Once the sequence was obtained from the literature, we simply codon optimized it for E coli to maximize production.


Source

This comes from the organism Ralstonia eutropha, which naturally produces PHB. The sequence has been codon optimized for use in E coli.

References